A Study of Peptide-Water Interactions Through 2D- WATERGATE - NOESY and ROESY and 1D Off- resonance ROESY Experiments: Application to Lys- vasopressin

H. TOMS*a, I.P. GEROTHANASSISb and G.E. HAWKESa

aDepartment of Chemistry, Queen Mary and Westfield College, Mile End Road, London E1 4NS, U.K.; and bDepartment of Chemistry, University of Ioannina , Ioannina GR-45110, Greece.

*Presenting Author. Tel: 0044 1717753257 Fax: 00441819818745

E-mail: h.toms@qmw.ac.uk

Abstract

Specific hydration of the NH protons of the neurohypophyseal peptide hormone Lys-vasopressin was investigated by the combined use of 2D-WATERGATE-NOESY/ROESY and 1D off- resonance WATERGATE-ROESY experiments. The 1D off-resonance experiment has significant sensitivity advantages compared to 2D-WATERGATE NOESY/ROESY. However, selectivity of the 180^o RE-BURP shaped pulse is very critical and the significant mobility of the peptide might complicate the interpretation.