From the above it is evident that the one-dimensional off-resonance ROESY experiment has sensitivity advantages compared with the 2D-WATERGATE NOESY/ROESY experiments for studying hydration accessibility, at a molecular level, of peptide hormones where the problems of signal overlapping are relatively minor. However, selectivity of the 180^o RE-BURP shaped pulse is very critical. We are currently investigating the 1D off-resonance ROE experiments in studying the solvation state of the small (~8%) but significant proportion of the cis isomer of Lys-vasopressin .

Acknowledgements

This research was supported from NATO (Collaborative Research Grant-960146) and the Research Committee of the University of Ioannina. The NMR spectra were obtained on the ULIRS Br\374ker AMX-600 facility located at QMW.