FIG.2. (a) 1D 1H NMR spectrum of Lys-vasopressin; (b)-(i) selective 1D off-resonance ROESY pulse sequence. (B) at different angles q using the same solution conditions to those of Fig. 1. A 100 ms, 7.8 KHz traperzoidal spin-lock pulse was applied during the mixing period; 16 dummy scans and scans were acquired for each experiment (interscan delay 5.9 s, total acquisition time 30 min). The water resonance was removed by the Bruker "qfil" routine and the baseline was improved by backwards linear prediction of the first 30 points.